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cd4 fitc labeled antibody  (Bio-Rad)


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    Bio-Rad cd4 fitc labeled antibody
    Cd4 Fitc Labeled Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd4 fitc labeled antibody/product/Bio-Rad
    Average 93 stars, based on 46 article reviews
    cd4 fitc labeled antibody - by Bioz Stars, 2026-03
    93/100 stars

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    Detection of the immune state of the TIME. (A) Schematic diagram of the administration and detection schedule. The proportion of (B,C) <t>CD4</t> + T cells and (D,E) CD8 + T cells of tumors from mice treated with NS, FA NAs, and FA-PTX NAs containing different doses of PTX at different time points as detected by flow cytometry. The numbers in the right area indicate the percentages of (B) CD4 + or (D) CD8 + T cells. (F) The distribution of CD8 + T cells in the TIME as detected by IHC. Scale bar =100 μm. (G) The concentration of TGF-β detected in tumors via ELISA in the different treatment groups at different time points. (H) The killing rate of splenic T cells as measured by lactate dehydrogenase release assay. FA-PTX NA, folic acid-modified nanoassemblies loaded paclitaxel; FA NA, folic acid-modified nanoassemblies; IHC, immunohistochemical; NS, normal saline; TIME, tumor immune microenvironment; TGF-β, transforming growth factor-beta.
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    Detection of the immune state of the TIME. (A) Schematic diagram of the administration and detection schedule. The proportion of (B,C) <t>CD4</t> + T cells and (D,E) CD8 + T cells of tumors from mice treated with NS, FA NAs, and FA-PTX NAs containing different doses of PTX at different time points as detected by flow cytometry. The numbers in the right area indicate the percentages of (B) CD4 + or (D) CD8 + T cells. (F) The distribution of CD8 + T cells in the TIME as detected by IHC. Scale bar =100 μm. (G) The concentration of TGF-β detected in tumors via ELISA in the different treatment groups at different time points. (H) The killing rate of splenic T cells as measured by lactate dehydrogenase release assay. FA-PTX NA, folic acid-modified nanoassemblies loaded paclitaxel; FA NA, folic acid-modified nanoassemblies; IHC, immunohistochemical; NS, normal saline; TIME, tumor immune microenvironment; TGF-β, transforming growth factor-beta.
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    Detection of the immune state of the TIME. (A) Schematic diagram of the administration and detection schedule. The proportion of (B,C) CD4 + T cells and (D,E) CD8 + T cells of tumors from mice treated with NS, FA NAs, and FA-PTX NAs containing different doses of PTX at different time points as detected by flow cytometry. The numbers in the right area indicate the percentages of (B) CD4 + or (D) CD8 + T cells. (F) The distribution of CD8 + T cells in the TIME as detected by IHC. Scale bar =100 μm. (G) The concentration of TGF-β detected in tumors via ELISA in the different treatment groups at different time points. (H) The killing rate of splenic T cells as measured by lactate dehydrogenase release assay. FA-PTX NA, folic acid-modified nanoassemblies loaded paclitaxel; FA NA, folic acid-modified nanoassemblies; IHC, immunohistochemical; NS, normal saline; TIME, tumor immune microenvironment; TGF-β, transforming growth factor-beta.

    Journal: Translational Lung Cancer Research

    Article Title: Nanoassemblies loaded with low-dose paclitaxel can enhance the response of lung cancer immunotherapy by activating dendritic cells

    doi: 10.21037/tlcr-2025-180

    Figure Lengend Snippet: Detection of the immune state of the TIME. (A) Schematic diagram of the administration and detection schedule. The proportion of (B,C) CD4 + T cells and (D,E) CD8 + T cells of tumors from mice treated with NS, FA NAs, and FA-PTX NAs containing different doses of PTX at different time points as detected by flow cytometry. The numbers in the right area indicate the percentages of (B) CD4 + or (D) CD8 + T cells. (F) The distribution of CD8 + T cells in the TIME as detected by IHC. Scale bar =100 μm. (G) The concentration of TGF-β detected in tumors via ELISA in the different treatment groups at different time points. (H) The killing rate of splenic T cells as measured by lactate dehydrogenase release assay. FA-PTX NA, folic acid-modified nanoassemblies loaded paclitaxel; FA NA, folic acid-modified nanoassemblies; IHC, immunohistochemical; NS, normal saline; TIME, tumor immune microenvironment; TGF-β, transforming growth factor-beta.

    Article Snippet: The cell suspensions were then stained with fluorochrome-labeled antibody-targeting murine CD4-FITC (eBioscience, San Diego, CA, USA), CD8-APC (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA), or an appropriate isotype control antibody and then analyzed by flow cytometry (Beckman Coulter, Brea, CA, USA).

    Techniques: Flow Cytometry, Concentration Assay, Enzyme-linked Immunosorbent Assay, Release Assay, Modification, Immunohistochemical staining, Saline

    Antitumor mechanism analysis. (A) HE staining of tumor tissues. Scale bar =100 μm. (B) The apoptosis induction as determined by TUNEL assay. Scale bar =50 μm. (C) The number of CD4 + and (D) CD8 + T cells in tumors detected by IHC. Scale bar =50 μm. (E) The distribution of CD8 + T cells in tumors detected by IHC. Scale bar =100 μm. (F-H) Tumor cell apoptosis and the abundance of CD4 + T cells and CD8 + T cells were assessed by counting the rate of TUNEL-positive cells and the number of CD4 + and CD8 + T cells, respectively. *, P<0.05; **, P<0.01; ***, P<0.001; ns, no significance. FA NA, folic acid-modified nanoassemblies; FA-PTX NA, folic acid-modified nanoassemblies loaded with paclitaxel; HE, hematoxylin and eosin; IHC, immunohistochemistry; NS, normal saline; PD-1, programmed cell death protein-1; TUNEL, terminal deoxynucleotidyl transferase 2'-deoxyuridine 5'-triphosphate nick end labeling.

    Journal: Translational Lung Cancer Research

    Article Title: Nanoassemblies loaded with low-dose paclitaxel can enhance the response of lung cancer immunotherapy by activating dendritic cells

    doi: 10.21037/tlcr-2025-180

    Figure Lengend Snippet: Antitumor mechanism analysis. (A) HE staining of tumor tissues. Scale bar =100 μm. (B) The apoptosis induction as determined by TUNEL assay. Scale bar =50 μm. (C) The number of CD4 + and (D) CD8 + T cells in tumors detected by IHC. Scale bar =50 μm. (E) The distribution of CD8 + T cells in tumors detected by IHC. Scale bar =100 μm. (F-H) Tumor cell apoptosis and the abundance of CD4 + T cells and CD8 + T cells were assessed by counting the rate of TUNEL-positive cells and the number of CD4 + and CD8 + T cells, respectively. *, P<0.05; **, P<0.01; ***, P<0.001; ns, no significance. FA NA, folic acid-modified nanoassemblies; FA-PTX NA, folic acid-modified nanoassemblies loaded with paclitaxel; HE, hematoxylin and eosin; IHC, immunohistochemistry; NS, normal saline; PD-1, programmed cell death protein-1; TUNEL, terminal deoxynucleotidyl transferase 2'-deoxyuridine 5'-triphosphate nick end labeling.

    Article Snippet: The cell suspensions were then stained with fluorochrome-labeled antibody-targeting murine CD4-FITC (eBioscience, San Diego, CA, USA), CD8-APC (Life Technologies, Thermo Fisher Scientific, Waltham, MA, USA), or an appropriate isotype control antibody and then analyzed by flow cytometry (Beckman Coulter, Brea, CA, USA).

    Techniques: Staining, TUNEL Assay, Modification, Immunohistochemistry, Saline, End Labeling